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Image Search Results


Journal: eLife

Article Title: Pathogen infection and cholesterol deficiency activate the C. elegans p38 immune pathway through a TIR-1/SARM1 phase transition

doi: 10.7554/eLife.74206

Figure Lengend Snippet:

Article Snippet: For purification, bacterial pellets were thawed on ice and then resuspended in Lysis Buffer (50 mM Tris•HCl pH 7.0, 300 mM NaCl, 10% (w/v) glycerol, 0.001% Tween 20) with Pierce EDTA-free protease inhibitor mini tablets (ThermoFisher Scientific).

Techniques: Protease Inhibitor, cDNA Synthesis, SYBR Green Assay, DC Protein Assay, DNA Purification, Recombinant, Plasmid Preparation, Software